Jane Poythress (1630 - 1676) was a member of the planter aristocracy in colonial Virginia.
Poythress was born arond 1630 in Jamestown, Virginia, as the daughter of Capt. Francis Poythress and Mary Sloman. She was a member of a famly known to have resided in the vicinity of Charles City, Virginia in the planter aristocracy.
Poythress died in 1676 in Charles City, Virginia. She was buried at Kippax Plantation in Virginia.
Poythress married Thomas Rolfe.